rabbit anti rat vegfr2 polyclonal antibody  (Boster Bio)

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on VEGFR2 protein expression in rat vascular endothelial cells. ( A ) Western blot bands of indicating proteins in endothelial cells; ( B ) densitometric analysis of the ratio of the intensity of protein bands. Values are the mean ± SD of VEGFR2 expression standardized to β-actin expression in three independent experiments; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on proliferation and migration of endothelial cells. ( A ) BrdU-positive cell rate; ( B ) migration distance statistics chart. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Migration

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on tube formation of endothelial cells. ( A ) Number of nodes forming a tube-like structure; ( B ) branch lengths forming the tube-like structure. Data expressed as mean ± SD; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the mouse matrigel plug model. ( A ) Pictures of the mouse matrigel plugs (a: PBS group, b: NC group, c: ROX group, d: sh-Vegfr2 group, and e: sh-Vegfr2 + ROX group); ( B ) hemoglobin content in matrigel plugs. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; # p < 0.05, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the growth and angiogenesis of/in melanoma xenografts. ( A ) Melanoma xenograft tumor volumes during the test period; ( B ) melanoma xenograft tumor weight at necropsy; ( C ) immunohistochemical expression of CD34 in melanoma xenografts. Data expressed as mean ± SD; * p < 0.05, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Expressing

Journal: BioMed Research International

Article Title: Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats

doi: 10.1155/2017/2597256

Figure Lengend Snippet: (a), (b), (c), (d) Immunohistochemical expression of VEGFR2 in epididymis caput of rats (SP ×400). (a) Control group, (b) low dose group, (c) middle dose group, and (d) high dose group.

Article Snippet: Sodium arsenite was obtained from Beijing Chemical Co. (pure analysis, Beijing Chemical Factory), cell apoptosis detection kit from Wuhan Boster Biotechnology Company, VEGF and VEGFR2 rabbit anti-rat polyclonal antibody from Wuhan Boster Biotechnology Company, SynGene Genius UV gel imaging system from Bio-Rad Company, USA, and Image J analysis software from Bio-Rad company, USA.

Techniques: Immunohistochemical staining, Expressing, Control

Journal: BioMed Research International

Article Title: Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats

doi: 10.1155/2017/2597256

Figure Lengend Snippet: (a), (b), (c), (d) Immunohistochemical expression of VEGFR2 in epididymis cauda of rats (SP ×400). (a) Control group, (b) low dose group, (c) middle dose group, and (d) high dose group.

Article Snippet: Sodium arsenite was obtained from Beijing Chemical Co. (pure analysis, Beijing Chemical Factory), cell apoptosis detection kit from Wuhan Boster Biotechnology Company, VEGF and VEGFR2 rabbit anti-rat polyclonal antibody from Wuhan Boster Biotechnology Company, SynGene Genius UV gel imaging system from Bio-Rad Company, USA, and Image J analysis software from Bio-Rad company, USA.

Techniques: Immunohistochemical staining, Expressing, Control

Journal: BioMed Research International

Article Title: Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats

doi: 10.1155/2017/2597256

Figure Lengend Snippet: Immunohistochemistry image analysis results of VEGFR2 protein in arsenic infected epididymis ( n = 10, x ± s).

Article Snippet: Sodium arsenite was obtained from Beijing Chemical Co. (pure analysis, Beijing Chemical Factory), cell apoptosis detection kit from Wuhan Boster Biotechnology Company, VEGF and VEGFR2 rabbit anti-rat polyclonal antibody from Wuhan Boster Biotechnology Company, SynGene Genius UV gel imaging system from Bio-Rad Company, USA, and Image J analysis software from Bio-Rad company, USA.

Techniques: Immunohistochemistry, Infection, Control

Journal: BioMed Research International

Article Title: Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats

doi: 10.1155/2017/2597256

Figure Lengend Snippet: Effects of arsenic poisoning on protein levels of VEGF and VEGFR2 in epididymis of rats ( n = 3, x ± s).

Article Snippet: Sodium arsenite was obtained from Beijing Chemical Co. (pure analysis, Beijing Chemical Factory), cell apoptosis detection kit from Wuhan Boster Biotechnology Company, VEGF and VEGFR2 rabbit anti-rat polyclonal antibody from Wuhan Boster Biotechnology Company, SynGene Genius UV gel imaging system from Bio-Rad Company, USA, and Image J analysis software from Bio-Rad company, USA.

Techniques: Control

Journal: BioMed Research International

Article Title: Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats

doi: 10.1155/2017/2597256

Figure Lengend Snippet: Effects of arsenic poisoning on mRNA levels of VEGF and VEGFR2 in epididymis of rats ( n = 3, x ± s).

Article Snippet: Sodium arsenite was obtained from Beijing Chemical Co. (pure analysis, Beijing Chemical Factory), cell apoptosis detection kit from Wuhan Boster Biotechnology Company, VEGF and VEGFR2 rabbit anti-rat polyclonal antibody from Wuhan Boster Biotechnology Company, SynGene Genius UV gel imaging system from Bio-Rad Company, USA, and Image J analysis software from Bio-Rad company, USA.

Techniques: Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: VEGFR2 + PDGFRβ + circulating precursor cells participate in capillary restoration after hyperoxia acute lung injury (HALI)

doi: 10.1111/j.1582-4934.2009.00785.x

Figure Lengend Snippet: Phenotypic characterization of CPCs. LSR-II flow cytometric analyses of (gated, a) mononuclear cells in peripheral blood, identified a distinct population of CPCs that co-express VEGFR2 and PDGFRβ (c); single-colour controls were used for compensation ( e.g. see staining for PDGFRβ-Alexa Fluor 647 in b). These CPCs (in blue) are positive for CD45 (d) and show scattering properties typical of small cells with a high nucleus to cytoplasm ratio (a, d). A subset of CPCs is positive for CD11b (e), whereas all VEGFR2 + PDGFRβ + CPCs are positive for CD31 (f); CPCs are distinct from cells with a phenotype consistent with ‘circulating ECs’, i.e. CD31 + CD45 − (see gated population in f).

Article Snippet: All Unicryl sections were pre-treated with 1% bovine serum albumin (BSA) in PBS (5 min at RT), incubated (overnight at 4°C) with ( i ) mouse anti-rat monoclonal antibody to CD11b (Calbiochem, La Jolla, CA, USA); ( ii ) rabbit anti-rat polyclonal antibodies to VEGFR2 or PDGFRβ (Calbiochem); or ( iii ) a rabbit polyclonal antibody to von Willebrand factor (vWF, DAKO, Carpinteria, CA, USA), rinsed in PBS, and incubated (60 min at RT) in protein A-gold (pA-AU) (10-nm diameter, Auroprobe AG10, Amersham, UK) diluted 1:50 in PBS prior to double-staining.

Techniques: Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: VEGFR2 + PDGFRβ + circulating precursor cells participate in capillary restoration after hyperoxia acute lung injury (HALI)

doi: 10.1111/j.1582-4934.2009.00785.x

Figure Lengend Snippet: Phenotypic characterization of CPCs in lungs post-HALI. Antigenic sites, visualized with 10-nm protein A-gold by high-resolution microscopy, demonstrated that CPCs were VEGFR2 + (a) and PDGFRβ + (b) post-HALI (weeks 6 and 7). Representative images of the same CPC profile in adjacent sections of lung tissue (c and d, and insets) demonstrated co-expression of VEGFR2 and PDGFRβ antigenic sites post-HALI (week 7). Other representative images of additional antigenic sites expressed by CPCs post-HALI (week 7): CD11b (e and inset) and for vWF (f). Typically, the sites for CD11b were uniformly distributed over CPCs but also appeared as clusters (e, see bottom inset, illustrating an example of a cluster of ∼40 CD11b + labelled sites). Ten-nm gold-labelled antigenic sites (a, c–f) are circled for clarity to distinguish these from 7 to 8 nm ribosomes on cell profiles. Ninety-nm-thick Unicryl resin sections stained with uranyl acetate and lead citrate. Bars = 0.5 μm (a–f).

Article Snippet: All Unicryl sections were pre-treated with 1% bovine serum albumin (BSA) in PBS (5 min at RT), incubated (overnight at 4°C) with ( i ) mouse anti-rat monoclonal antibody to CD11b (Calbiochem, La Jolla, CA, USA); ( ii ) rabbit anti-rat polyclonal antibodies to VEGFR2 or PDGFRβ (Calbiochem); or ( iii ) a rabbit polyclonal antibody to von Willebrand factor (vWF, DAKO, Carpinteria, CA, USA), rinsed in PBS, and incubated (60 min at RT) in protein A-gold (pA-AU) (10-nm diameter, Auroprobe AG10, Amersham, UK) diluted 1:50 in PBS prior to double-staining.

Techniques: Microscopy, Expressing, Staining